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Collecting tissue

A growing need is anticipated for large collections of well-diagnosed fresh frozen tumor tissue.

Tissues are obtained from left over tissues from patients, originating from surgical resection specimens and sometimes biopsies from patients. The tissues are presented to the pathology department for diagnostic purposes. However, in most cases not all tissue is needed for diagnosis and the “left over tissue”  can be used for research purposes. Scientific research on patient material has to comply to strict rules and regulations

If available, tumor tissue, corresponding pre-malignant and normal tissue samples are collected. Before freezing the obtained sample is divided in two, only if the sample appears large enough. One part is reserved for freezing and the other for paraffin embedding. Freezing and embedding is done oriented in such a way that the first tissue slide shares the plane of the original dividing cut. To preserve histologic quality of future frozen tissue slides the tissue samples are snap frozen in liquid nitrogen pre-cooled iso-pentane. Oriented freezing is performed on a flat piece of cork covered with filter paper wetted with physiologic salt solution. The paraffin embedded tissue is used to make routine Heamatoxilin/Eosin (HE) stained slides. The prepared slides are used for the acquisition of the digital histologic images and quality control. Quality control is performed by an expert pathologist and an employee of the Tissue Bank, on all new samples since the beginning of 2003. They compare the clinical diagnosis with the content of the tissue slide. This fast form of diagnostics is kindly provided by the Pathologists Michael den Bakker and Ronald de Krijger. Aberrant findings are added to the remarks section of the database.

Such a collection would enable high quality DNA, RNA and protein isolation from well documented frozen tissues accompanied by a solid diagnosis. To facilitate easy search for the availability of tissues for research projects the generation of an anonymous database containing documentation of the frozen tissue samples with as many attributes as possible is inevitable. In addition, the database contains digital histologic images of the frozen specimen to enable good evaluation of the groups acquired from searching the database, before retrieving the valuable tissue specimen from the liquid nitrogen storage. The images will be provided in two magnifications, 100x (overview) and 400x (specific). 

Tissue types:

Brain                            -    collected by others
Ear Nose Throat  
Testis/germ cell tumors  -    collected by others