Pre-Klinisch

 

Kwaliteit orale weefsels na radiotherapie hoofd-/halsgebied

  

Korte beschrijving:

  

Radiotherapie wordt algemeen toegepast bij de behandeling van kwaadaardige tumoren in het hoofd/halsgebied. Behalve tot aantasting van tumorweefsel leidt radiotherapie ook tot beschadiging van omringende weefsels, waardoor reconstructieve chirurgische behandelingen worden belemmerd. Bij patiƫnten die operatief en/of radiotherapeutisch behandeld zijn voor hoofd/halstumoren wordt vaak hyperbare zuurstof (HBO) therapie toegepast om regeneratie en reparatie van beschadigd weefsel te stimuleren. In onze preklinische studies wordt de invloed van HBO therapie op bestraalde en onbestraalde orale weefsels bestudeerd. Tevens wordt onderzocht wat het effect van HBO therapie is op de groei en ontwikkeling van residuaal (bestraald) tumorweefsel. Dit onderzoek heeft een NUTS/Ohra subsidie.

  

Recente publicaties:

  1. A hyperbaric oxygen chamber for animal experimental purposes. Djasim UM, Spiegelberg L, Wolvius EB, Wal KG van der. International Journal of Oral and Maxillofacial Surgery 2012; 41(2):271-274
  2. The effects of heparan sulphate mimetic RGTA-OTR4120 on irradiated murine salivary glands. Spiegelberg L, Djasim UM, Neck JW van, Wolvius EB, Wal KG van der . Journal of Oral Pathology and Medicine 2012;41(6):477-483
  3. Hyperbaric oxygen therapy in the management of radiation-induced injury in the head and neck region: a review of the literature. Spiegelberg L, Djasim UM, Neck JW van, Wolvius EB, Wal KG van der. Journal of Oral and Maxillofacial Surgery 2010;68(8):1732-9

Naam onderzoekers: Linda Spiegelberg/Anneke Braks

  

Functie: wetenschappelijk onderzoekers


Elucidation of donor-host interactions..

  

Korte beschrijving:

 

Elucidation of donor-host interactions using adult mesenchymal stem cells for the scale-up of bone tissue engineering by endochondral ossification.

The aim of my research is to better understand how adult MSCs generate bone by endochondral ossification and to identify the role of the host in order to generate a new bone replacement technique/technology. Current bone tissue engineering approaches have not yet satisfied the unmet need for replacement bone tissue. The main reasons for failure of implants, both tissue engineered or otherwise are lack of proper vascularisation of the implant and poor integration with the surrounding host tissue. I have shown that adult mesenchymal stem cells (MSCs), following brief chondrogenic priming in vitro, can form mature, vascularised, marrow-containing bone in vivo. The exact mechanisms by how this occurs are not known. Furthermore, quantities of bone formed by this approach have thus far been no more than a few cubic millimetres in size. My research will address these issues by i) investigating in detail the interactions between implanted MSCs and the host ii) developing a novel tissue engineering based approach to bone formation and repair utilising donor host interactions and MSC paracrine activities. By developing a greater understanding of how MSCs form bone in vivo, translating that information into a novel application and testing that application in relevant models I will generate knowledge and outputs that will lead to the creation of new therapies for large bone defects.

 

Naam onderzoeker: Eric Farrell

 

Functie: Assistant professor/ Principal Investigator