Sonja Buschow

PhD

Sonja Buschow 0416-013Sonja Buschow,
PhD
Room Na-619
E-mail: s.buschow@erasmusmc.nl

From 1996-2002 Sonja Buschow studied Medical Biology at Utrecht University. For her Master degree she studied GGA protein function in the group of prof. Margaret Robinson at the University of Cambridge (United Kindom) and MHC class II sorting into Multi-vesicular bodies in the Laboratory of prof. Willem Stoorvogel at the UMC Utrecht. In the latter research group she continued her scientific training studying the mechanisms of formation, secretion and targeting of Dendritic cell (DC) and B cell exosomes.
After receiving her doctorate in 2006 she moved to the department of tumor Immunology at the UMC St Radboud/ NCMLS to work as a postdoctoral scientist in the group of prof. Carl Figdor. Here she used Mass spectrometry and transcriptome analysis to study the molecular dynamics of phagosomes, DCs and blood cells to learn more about the optimal application of dendritic cells in cancer immunotherapy. This resulted in several publications describing the proteome of phagosomes and DCs and in the discovery of Semaphorin 7A as a regulator of DC migration.  In 2011 she was granted an EMBO-short term fellowship for a 3-month visit to the laboratory of Prof Garcia-Parajo at the Institute of photonic sciences in Barcelona (Spain) to use high resolution microscopy to study cell surface organization of the pattern recognition receptor DC-SIGN in DCs.  
In 2014 Sonja continued her scientific career at her current location in the department of Gastroenterology to work with Dr. Andrea Woltman and to study antigen presentation in chronic hepaptitis B (HBV) and HBV related hepatocellular carcinoma. Her final goal is to develop antigen presentation based immunotherapy to fight these deadly conditions for which currently only limited treatment options exist. For therapy design she now studies the presentation of (viral) antigens on dendritic cells and hepatocytes and investigates DC function and T cell responses in HBV related liver disease.

        
Pubmed for S. Buschow

Selected publications:

  • Worah K, Mathan TS, Vu Manh TP, Keerthikumar S, Schreibelt G, Tel J, Duiveman-de Boer T, Sköld AE, van Spriel AB, de Vries IJ, Huynen MA, Wessels HJ, Gloerich J, Dalod M, Lasonder E, Figdor CG, Buschow SI.
    Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function.
    Cell Rep. 2016 Sep 13;16(11):2953-66.
  • van der Aa E, Buschow SI, Biesta PJ, Janssen HL, Woltman AM.
    The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function.
    PLoS One. 2016 Aug 16;11(8):e0161235.
  • Bakdash G, Buschow SI, Gorris MA, Halilovic A, Hato SV, Sköld AE, Schreibelt G, Sittig SP, Torensma R, Duiveman-de Boer T, Schröder C, Smits EL, Figdor CG, de Vries IJ.
    Expansion of a BDCA1+CD14+ Myeloid Cell Population in Melanoma Patients May Attenuate the Efficacy of Dendritic Cell Vaccines.
    Cancer Res. 2016 Aug 1;76(15):4332-46.
  • van Montfoort N, van der Aa E, van den Bosch A, Brouwers H, Vanwolleghem T, Janssen HL, Javanbakht H, Buschow SI, Woltman AM.
    Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism.
    J Virol. 2016 Jun 24;90(14):6187-99.
  • Eich C, Lasonder E, Cruz LJ, Reinieren-Beeren I, Cambi A, Figdor CG, Buschow SI.
    Proteome Based Construction of the Lymphocyte Function-Associated Antigen 1 (LFA-1) Interactome in Human Dendritic Cells.
    PLoS One. 2016 Feb 18;11(2):e0149637.
  • van Rijn A, Paulis L, te Riet J, Vasaturo A, Reinieren-Beeren I, van der Schaaf A, Kuipers AJ, Schulte LP, Jongbloets BC, Pasterkamp RJ, Figdor CG, van Spriel AB, Buschow SI.
    Semaphorin 7A Promotes Chemokine-Driven Dendritic Cell Migration.
    J Immunol. 2016 Jan 1;196(1):459-68.
  • Buschow SI, Sprengers D, Woltman AM.
    To target or not to target viral antigens in HBV related HCC?
    J Hepatol. 2015 Jun;62(6):1449-50.
  • Buschow SI, Lasonder E, Szklarczyk R, Oud MM, de Vries IJ, Figdor CG.
    Unraveling the human dendritic cell phagosome proteome by organellar enrichment ranking.
    J Proteomics. 2012 Feb 16;75(5):1547-62.
  • Buschow SI, Lasonder E, van Deutekom HW, Oud MM, Beltrame L, Huynen MA, de Vries IJ, Figdor CG, Cavalieri D.
    Dominant processes during human dendritic cell maturation revealed by integration of proteome and transcriptome at the pathway
    level.
    J Proteome Res. 2010 Apr 5;9(4):1727-37.
  • Buschow SI, Nolte-'t Hoen EN, van Niel G, Pols MS, ten Broeke T, Lauwen M, Ossendorp F, Melief CJ, Raposo G, Wubbolts R, Wauben MH, Stoorvogel W.
    MHC II in dendritic cells is targeted to lysosomes or T cell-induced exosomes via distinct multivesicular body pathways.
    Traffic. 2009 Oct;10(10):1528-42.