Pothof, Joris

Joris Pothof studied Biology (specialty Molecular Cell Biology and Genetics) at Leiden University, the Netherlands. He received his PhD in Medicine from Utrecht University on the basis of a research project conducted at the Hubrecht laboratory in the group of Dr. R. Plasterk. Here he studied DNA damage responses in the nematode C. elegans. He performed his post-doctoral training at the Erasmus MC Genetics department, studying DNA damage response networks in aging and cancer using genomics with a focus on RNA biology. Subsequently, he started his own research group at the Genetics Department continuing his post-doctoral research.

Current research interests focus on:
• Mapping DNA damage response networks using state-of-the-art genomics and proteomics technologies followed by functional characterization.
• Developing novel genomics methodologies for next generation sequencing.
• The role of DNA damage and DNA damage response networks in aging and age-related diseases.
• The role of DNA damage response networks in cancer therapy resistance.
To unravel these complex DNA damage response networks we apply an integrated multi-disciplinary approach using a variety of research tools, including genomics/proteomics, mouse-models, organotypic tissue slices and organoids, molecular/cellular biology, biochemistry and microscopy.

Selected Publications.
Deciphering the RNA landscape by RNAome sequencing.
Derks KW, Misovic B, van den Hout MC, Kockx CE, Gomez CP, Brouwer RW, Vrieling H, Hoeijmakers JH, van IJcken WF, Pothof J. Deciphering the RNA landscape by RNAome sequencing. RNA Biol. 2015 Jan 2;12(1):30-42. doi: 10.1080/15476286.2015.1017202.

Vermeij WP, Hoeijmakers JH, Pothof J. Aging: not all DNA damage is equal. Curr Opin Genet Dev. 2014 Jun;26:124-30. doi: 10.1016/j.gde.2014.06.006. Epub 2014 Sep 15.

van Jaarsveld MT, Wouters MD, Boersma AW, Smid M, van Ijcken WF, Mathijssen RH, Hoeijmakers JH, Martens JW, van Laere S, Wiemer EA, Pothof J. DNA damage responsive microRNAs misexpressed in human cancer modulate therapy sensitivity. Mol Oncol. 2014 May;8(3):458-68. doi: 10.1016/j.molonc.2013.12.011. Epub 2013 Dec 31.

Pothof J, Verkaik NS, van IJcken W, Wiemer EA, Ta VT, van der Horst GT, Jaspers NG, van Gent DC, Hoeijmakers JH, Persengiev SP. MicroRNA-mediated gene silencing modulates the UV-induced DNA-damage response. EMBO J. 2009 Jul 22;28(14):2090-9. doi: 10.1038/emboj.2009.156. Epub 2009 Jun 18.


DNA damage response
Cancer therapy resistance
Next Generation Sequencing