Project 15

Role of hemostasis in arterial thrombosis

The studies performed within this theme focus on the role of coagulation factors and platelets in the development of arterial thrombosis. Several case-control studies have been initiated to investigate the role of haemostatic or inflammatory factors in determining the risk of first and recurrent arterial thrombotic events, including stroke and acute coronary syndrome. Several genetic approaches including GWAS, SNP and haplotype analysis are regularly applied to test associations with von Willebrand factor and fibrinolysis. Our most recent research focuses on ADAMTS13, the von Willebrand factor cleaving protease, of which plasma levels are associated with the risk of cardiovascular disease. We have obtained more insight in the mechanism of fibrin formation and fibrinolysis by identifying new proteins binding to fibrin using plasma proteomics techniques, specifically the Neutrophil Extracellular Traps (NETs). The effect of fibrin structure on the risk of thrombosis and the relationship with atherosclerosis and angiogenesis, is studied using recombinant and plasma-purified fibrinogen forms.

Principal InvestigatorsFWG Leebeek, MPM de Maat, DC Rijken
Co-Investigators JW Deckers, DWJ Dippel, OH Franco, M Kavousi, PJ Koudstaal
PostdocS Uitte de Willige, S Talens
PhD candidatesT. Hoppenbrouwers, A.S.A. Autar, A. Dreijer, F.N. Croles, S. Abdul, S. Donse-Donkel